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Shokoofeh Hajihashemi Khadijeh Kiarostami Azra Saboora Shokoofeh Enteshari 《Plant Growth Regulation》2007,53(2):117-128
Effect of paclobutrazol (PBZ) treatment on salinity tolerance of wheat (Triticum aestivum) was investigated on a salt-tolerant (Karchia-65) and salt-sensitive (Ghods) cultivars. Salinity significantly reduced the
investigated growth parameters such as plant height, length and area of sixth leaf, root length, fresh and dry weight of shoot,
roots and sixth leaf, water content (WC) of plant and seeds weight in the both cultivars. The negative effect of salinity
in Ghods cultivar was more than Karchia cultivar. However, PBZ treatment reduced the growth in both cultivars, the differences
in plant growth among various levels of NaCl decreased in PBZ-treated plants. Salt stress resulted in high accumulation of
Na+ in the sixth leaf and roots in both cultivars, particularly in Ghods cultivar. Against Karchia cultivar, salt stress decreased
the storage of K+, P and N in sixth leaf and roots in Ghods cultivar. In the both cultivars, PBZ treatment enhanced the K+, P and N contents in sixth leaf and roots by increasing salinity. Although PBZ treatment decreased the growth of plants,
it improved the weight of seeds against stress damage. PBZ treatment reduced the accumulation of harmful Na+ ion in plant tissues while increased the K+, P and N contents. These observations suggest that PBZ treatment may increase tolerance by diminishing ionic imbalance caused
by salt stress. 相似文献
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Ghomi Khadijeh Rabiei Babak Sabouri Hossein Gholamalipour Alamdari Ebrahim 《Molecular biology reports》2021,48(10):6673-6694
Molecular Biology Reports - Barley is one of the major cereal crops, which can provide a significant source of genes for stress tolerance due to its high diversity and adaptability. Metabolite... 相似文献
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Khadijeh Khanaliha Mohammad Hossein Motazedian Bahram Kazemi Bahador Shahriari Mojgan Bandehpour Zarin Sharifniya 《The Korean journal of parasitology》2014,52(2):137-142
Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii. 相似文献
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The phonon and thermal properties of different single- ((n,0) (n = 7,8,9,10,14,15)) and double-walled carbon nanotubes ((7, 0) @ (14, 0), (8, 0) @ (14, 0), (9, 0) @ (15, 0) and (10, 0) @ (15, 0),) were calculated using the combination of density functional theory and non-equilibrium Green’s function methods. It was found that the Seebeck and Peltier coefficients for some of the single- and multi-walled carbon nanotubes have negative values. Moreover, in sharp contrast to low ?T, the higher thermoelectric figure of merit is anticipated at the higher temperature. The effect of the atoms number per unit cell on the phonons energies outweighs the effect of the vacuum and the size of the tubes for DWCNTs. All in all, the electron–phonon coupling generates the roughly plethora of thermoelectric coefficients and thermal conductance. 相似文献
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A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry 下载免费PDF全文
Khadijeh Rajabi Julia Reuther Elke Deuerling Sheena E Radford Alison E Ashcroft 《Protein science : a publication of the Protein Society》2015,24(8):1282-1291
The kinetics and thermodynamics of protein folding are commonly studied in vitro by denaturing/renaturing intact protein sequences. How these folding mechanisms relate to de novo folding that occurs as the nascent polypeptide emerges from the ribosome is much less well understood. Here, we have employed limited proteolysis followed by mass spectrometry analyses to compare directly free and ribosome-tethered polypeptide chains of the Src-homology 3 (SH3) domain and its unfolded variant, SH3-m10. The disordered variant was found to undergo faster proteolysis than SH3. Furthermore, the trypsin cleavage patterns observed show minor, but significant, differences for the free and ribosome-bound nascent chains, with significantly fewer tryptic peptides detected in the presence of ribosome. The results highlight the utility of limited proteolysis coupled with mass spectrometry for the structural analysis of these complex systems, and pave the way for detailed future analyses by combining this technique with chemical labeling methods (for example, hydrogen-deuterium exchange, photochemical oxidation) to analyze protein folding in real time, including in the presence of additional ribosome-associated factors. 相似文献
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